Journal: Translational Cancer Research

Article Title: Long non-coding RNA (LncRNA) CHROMR promotes the expression of the CNNM1 gene by adsorbing hsa-miR-1299 to obtain drug resistance in diffuse large B lymphoma cells

doi: 10.21037/tcr-22-1087

Figure Lengend Snippet: CHROMR’s role in the DLBCL cell line. (A) After CHROMR overexpression, detect CHROMR, miR-1299, and CNNM1 expression changed in SU_DHL_4 cell line by RT-qPCR. (B) After CHROMR overexpression, we detected apoptosis-related genes and CNNM1 expression by Western Blot. (C) In the case of rituximab killing, flow cytometry detected the proportion of cells in the G2 phase of each group. (D) In the case of rituximab killing, the proportion of apoptosis in each group was detected by flow cytometry. (E) After CHROMER overexpression, we detected cell proliferation ability of each group by cck8. *, P<0.05; ***, P<0.001. NC, negative control; DLBCL, diffuse large B-cell lymphoma; RT-qPCR, real time quantitative polymerase chain reaction.

Article Snippet: When necessary, 17 nM rituximab (MCE) was added to the medium to induce cell death.

Techniques: Over Expression, Expressing, Quantitative RT-PCR, Western Blot, Flow Cytometry, Negative Control, Real-time Polymerase Chain Reaction

Journal: Translational Cancer Research

Article Title: Long non-coding RNA (LncRNA) CHROMR promotes the expression of the CNNM1 gene by adsorbing hsa-miR-1299 to obtain drug resistance in diffuse large B lymphoma cells

doi: 10.21037/tcr-22-1087

Figure Lengend Snippet: Verification of the CHROMR/miR-1299/CNNM1 pathway through cell function, RT-qPCR, and Western Blot experiments. (A) After transfection of CHROMR-over plasmid and miR-1299 mimic recovery, we detected CHROMR, miR-1299, CNNM1 expression changes in SU_DHL_4 cell line by RT-qPCR. (B) After transfection of CHROMR-over plasmid and miR-1299 mimic recovery, we detected apoptosis-related genes and CNNM1 expression by Western Blot. + indicates that the substance is transfected, − indicates that the substance is not transfected. (C) In the case of rituximab killing, flow cytometry detected the proportion of cells in the G2 phase of each group. (D) In the case of rituximab killing, the proportion of apoptosis in each group was detected by flow cytometry. ***, P<0.001. NC, negative control; RT-qPCR, real time quantitative polymerase chain reaction.

Article Snippet: When necessary, 17 nM rituximab (MCE) was added to the medium to induce cell death.

Techniques: Cell Function Assay, Quantitative RT-PCR, Western Blot, Transfection, Plasmid Preparation, Expressing, Flow Cytometry, Negative Control, Real-time Polymerase Chain Reaction

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Effects of the Higenamine, a Potent Compound from Aconitum , on UVB-Induced Photoaging in Hairless Mice

doi: 10.1155/2022/9116642

Figure Lengend Snippet: Promotive effect of higenamine on the collagen-related proteins. The levels of TGF- β (a), Smad3 (b), and COL1A1 (c) determined by ELISA. Values are expressed as the mean ± standard error of the mean. ∗ P < 0.05 and ∗∗ P < 0.01 compared to the control group.

Article Snippet: The COL1A1 ELISA kit was obtained from Cusabio Technology (Wuhan, China).

Techniques: Enzyme-linked Immunosorbent Assay, Control

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Effects of the Higenamine, a Potent Compound from Aconitum , on UVB-Induced Photoaging in Hairless Mice

doi: 10.1155/2022/9116642

Figure Lengend Snippet: Effect of higenamine on UVB-induced photoaging in the skin of mice. Representative histological analysis of skin section damaged by UVB exposure. (a) Masson's trichrome staining to identify collagen fibers. The levels of Smad3 (b) and COL1A1 (c) determined by ELISA. Values are expressed as the mean ± standard error of the mean. ∗∗ P < 0.01 and ∗∗∗ P < 0.001 compared to the control group, and ## P < 0.01 and ### P < 0.001 compared to the UVB-alone group.

Article Snippet: The COL1A1 ELISA kit was obtained from Cusabio Technology (Wuhan, China).

Techniques: Staining, Enzyme-linked Immunosorbent Assay, Control

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Effects of the Higenamine, a Potent Compound from Aconitum , on UVB-Induced Photoaging in Hairless Mice

doi: 10.1155/2022/9116642

Figure Lengend Snippet: Effect of higenamine on the levels of UVB-induced photoaging in vitro. Cytotoxicity levels measured by MTT (a), and COL1A1 levels measured by ELISA (b) in cells treated with higenamine followed by UVB stimulation. Levels of Smad2 DNA-binding Phosphorylation (c) and COL1A1 (d) in TGF- β siRNA-transfected differentiated human primary fibroblast cells. Values are expressed as the mean ± standard error of the mean. # P < 0.05 and ### P < 0.001 compared to the control group, ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001 compared to the UVB-alone group, & P < 0.05 compared to the si-TGF- β group, and @ P < 0.05 compared to the higenamine + si-TGF- β + UVB group.

Article Snippet: The COL1A1 ELISA kit was obtained from Cusabio Technology (Wuhan, China).

Techniques: In Vitro, Enzyme-linked Immunosorbent Assay, Binding Assay, Phospho-proteomics, Transfection, Control